Mmd how to use more than one mme effect
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With tumor necrosis factor alpha (TNF-α) being the key cytokine in inflammation, many anti-TNF therapies emerged and dominated in the treatment of CD 2, 3. Inflammatory bowel disease (IBD) includes both Crohn's disease (CD) and ulcerative colitis (UC) and results from improper inflammatory response to microbiota in a host with genetic susceptibility 1.
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The present study confirmed MMD and ELOVL7 involvement in anti-TNF response and revealed that the regulation of MMD and ELOVL7 gene regions in ADA response may be a part of a complex interplay extending from genetic to epigenetic and to transcriptomic level. Functional analysis has shown that rs1465352, rs4422035 and rs78620886 are listed at H3K9ac_Pro histone modification epigenetic mark. Subsequently, analysis of single nucleotide variants in regions of confirmed genes identified 5 variants near MMD and two in ELOVL7 intronic regions associated with treatment response to anti-TNF. RNA-seq analysis confirmed 7 out of 65 previously suggested genes involved in anti-TNF response. Association regressions were carried out with 12 week response to adalimumab as an outcome variable. Genotyping was performed using Infinium Global Screening Array. DNA and RNA were extracted from peripheral blood mononuclear cells. CD patients, who were naïve with respect to the treatment with biologicals, were enrolled in the study. Here we integrated our and previously reported PBMC derived transcriptomic and genomic data for identification of biomarkers for discrimination between responders and non-responders to anti-TNF therapy. Response to anti-TNF therapy is of pivotal importance in patients with Crohn’s disease (CD).